|
KMID : 1036320150370010031
|
|
Maxillofacial Plastic and Reconstructive Surgery
2015 Volume.37 No. 1 p.31 ~ p.31
|
|
|
Combined effect of bisphosphonate and recombinant human bone morphogenetic protein 2 on bone healing of rat calvarial defects
|
|
Kim Ho-Chul
Song Jae-Min
Kim Chang-Joo
Yoon Sang-Yong
Kim In-Ryoung
Park Bong-Soo
Shin Sang-Hun
|
|
|
Abstract
|
|
|
Background: This study aimed to investigate new bone formation using recombinant human bone morphogenetic protein 2 (rhBMP-2) and locally applied bisphosphonate in rat calvarial defects.
Methods: Thirty-six rats were studied. Two circular 5 mm diameter bony defect were formed in the calvaria using a trephine bur. The bony defect were grafted with Bio-Oss® only (group 1, n?=?9), Bio-Oss® wetted with rhBMP-2 (group 2, n?=?9), Bio-Oss® wetted with rhBMP-2 and 1 mM alendronate (group 3, n?=?9) and Bio-Oss® wetted with rhBMP-2 and 10 mM alendronate (group 4, n?=?9). In each group, three animals were euthanized at 2, 4 and 8 weeks after surgery, respectively. The specimens were then analyzed by histology, histomorphometry and immunohistochemistry analysis.
Results: There were significant decrease of bone formation area (p?
Conclusion: Low concentration bisphosphonate and rhBMP-2 have synergic effect on bone regeneration and this is result from the decreased activity of RANKL of osteoblast.
|
|
|
KEYWORD
|
|
|
Bisphophonate, Bone morphogenetic protein 2, Bone regeneration, Histology, Alendronate
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|
|